The aim of this article is to provide information on environmental changes in the Targowisko region in the Early Neolithic as a natural response to settlement and economic activity of the human population in that area. The discussion is based on lithological, geochemical, and palynological analyses, as well as the analysis of Cladocera within strata inside the TRG (Targowisko) core, located in a small wetland in the immediate vicinity of the eastern edge of the Neolithic settlement in the Targowisko region. Settlement analysis points to the absence of stable microregions and to the mobility of human groups. This is confirmed by the sequence of settlement episodes and economic activity, reflected in the stratigraphy of the core sediments, where episodes of significant human interference are followed by phases of almost complete regeneration of the environment. No differences have been noticed between the Linienbandkeramik and Malice culture communities as regards their impact on the environment.