Digital technologies integrated into port logistics are becoming increasingly decisive among port cities around the world. This growing importance is due to the need for policymakers, urban managers, port authorities, local administrators, shipping companies, couriers, and so on to develop increasingly digitalized and sustainable logistic processes. Therefore, in a global context characterized by intense datafication and globalization of trade, the data-based approach has become a necessary modus operandi to promote smart and sustainable logistics development. This forward-looking model of port logistics uses technologies such as IoT, sensors, cloud computing platforms, Big Data analytics, Artificial Intelligence (AI), GPS tracking systems, radars, drones, real-time monitoring stations, smart grids, and so on in order to collect, process, monitor and analyse data and information concerning the economic, environmental, social and technological sphere of port cities. In this sense, mobile and fixed platforms help logistics operators to optimize the management of flows (e.g., water, waste, emissions, raw materials, people, monetary investments, etc.) in an efficient and digitized manner. The study proposes a systematic literature review of the most recurring themes concerning smart and sustainable logistics initiatives within port cities in order to develop a multidimensional framework capable of holistically integrating the prevailing enabling factors (Ecosystem, Internal Organization, Data and Security, Policy and Regulation, Finance and Funding, and Digital and Technology), domains (Mobility, Environment, Economy, Telecommunications, Safety and Security, Government, and Community) and goals (Sustainable Development and Digitalization) that characterize smart and sustainable logistical development. To this end, the best practices of several pioneering port cities such as Rotterdam, Hamburg, Singapore, Los Angeles, Amsterdam, etc. implemented in partnerships with technology companies such as Cisco, IBM, Huawei and SAP were also analysed. Therefore, the results of this research show that smart and sustainable logistics initiatives in port cities: (a) have the potential to enhance the efficiency of the economic, environmental, social and technological flows; (b) increase the involvement and awareness of stakeholders such as couriers, shippers, shipping companies, citizens, port authorities, municipalities, security officers, gate and terminal personnel, and so on; and (c) provide a detailed overview of the enabling factors, domains and goals that must be activated by port cities to foster a smart and sustainable logistic transition.