The aim of the study was to evaluate the effect of maximal specific physical effort on postural balance and rifle stability in biathletes in a standing shooting position. The study included 10 junior elite biathletes. The measurements were taken with the Vicon system and AMTI force platform. Postural balance and rifle stability characteristics were determined at rest as well as 1 and 5 min post maximal specific physical effort which was performed on a ski ergometer and continued until exhaustion. Maximal physical effort exerted a significant effect on all examined postural sway and rifle sway characteristics. The duration of the post-exercise changes was longer than 5 minutes. Higher post-effort rifle sway was observed in the vertical direction than in the across the shooting line direction. Post-effort postural balance impairment in the shooting line was much greater than in the across the shooting line direction. Moreover, a strong correlation was found between postural balance and rifle stability. Maximal physical effort influenced postural balance and rifle stability during aiming. Rifle sway during aiming in a standing shooting position seems to be coordinated with the postural sway of the biathlete’s body. Thus, an increase in postural sway contributes to greater sway and lesser stability of the rifle.